The solubility of this product in DMSO has not been tested. A 200 mM stock solution can be prepared using ethanol, methanol, and 2-propanol. It is known to have 100 mM solubility in DMSO. The product is insoluble in aqueous solutions. Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C. Storing in a desiccator may be useful.
P7626
Fluoruro de fenilmetansulfonilo
powder, ≥98.5% (GC)
Sinónimos:
αFluoruro de α-toluenosulfonilo, Fluoruro de bencilsulfonilo, Fluoruro de fenilmetilsulfonilo, PMSF
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About This Item
Fórmula empírica (notación de Hill):
C7H7FO2S
Número de CAS:
Peso molecular:
174.19
Beilstein/REAXYS Number:
2088311
EC Number:
MDL number:
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.77
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Nombre del producto
Fluoruro de fenilmetansulfonilo, ≥98.5% (GC)
biological source
synthetic
Quality Level
assay
≥98.5% (GC)
form
powder
mp
91-94 °C
solubility
dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.)
H2O: unstable
SMILES string
FS(=O)(=O)Cc1ccccc1
InChI
1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
InChI key
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
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General description
El fluoruro de fenilmetanosulfonilo (PMSF) es un inhibidor de las serín-proteasas muy utilizado, eficaz contra enzimas como la quimiotripsina, la trombina y la tripsina. El PMSF actúa como inhibidor mediante la sulfonación de los restos de hidroxilo de los residuos de serina en los sitios reactivos de las proteasas de serina.
Application
El PMSF se utiliza a menudo en el tampón de lisis para contribuir a preservar las proteínas de interés durante el aislamiento de las proteínas y la preparación de las muestras inhibiendo las proteasas que, de otro modo, degradarían las proteínas después de la lisis celular o tisular. El PMSF es inestable en medios acuosos. Por consiguiente, las disoluciones madre de PMSF se preparan generalmente en disolventes orgánicos anhidros (por ejemplo etanol al 100 % o isopropanol anhidro) antes de su uso en medios acuosos.
Las características y beneficios generales observados del PMSF son:
Las características y beneficios generales observados del PMSF son:
- Inhibe las proteasas de serina como la tripsina y la quimiotripsina
- Inhibe también las proteasas de cisteína (reversible por tioles reducidos) y la acetilcolinesterasa de mamíferos
- No es tan eficaz ni tan tóxico como el DFP
- Concentración efectiva 0.1-1 mM
- Semivida = 1 h a pH 7,5
El fluoruro de fenilmetanosulfonilo se ha utilizado en las siguientes aplicaciones:
Biochem/physiol Actions
La administración de PMSF produce analgesia no relacionada con su efecto anticolinesterásico y prolonga el efecto analgésico de la β-endorfina administrada centralmente.[5]
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Referencia del producto
Descripción
Precios
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Oral - Skin Corr. 1B
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Protocolos
Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
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Can this PMSF powder be dissolved in DMSO, and if yes, then to what concentration where and how to store the reconstituted vial?
1 answer-
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At what temperature should the stock Product P7626, Phenylmethanesulfonyl fluoride (PMSF), be stored?
1 answer-
A 200 mM PMSF solution in dry solvent has been reported to remain active for at least 9 months at
2-8 °C. 100 mM PMSF solutions in isopropanol may be stored at –20 °C for several months. For more information please view the Product Data Sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/348/427/p7626pis-ms.pdfHelpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the effective amount of Product P7626, Phenylmethanesulfonyl fluoride (PMSF), to be used?
1 answer-
The effective concentration range is 0.1-1 mM. A final concentration of 5 mM was used for proteinase K inhibition.
Helpful?
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How should stock solutions of Product P7626, Phenylmethanesulfonyl fluoride (PMSF), be stored?
1 answer-
A 200 mM solution in dry solvent is stable for at least 9 months when stored at 2-8°C.
Helpful?
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What does Product P7626, Phenylmethanesulfonyl fluoride (PMSF), inhibit?
1 answer-
PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases.
Helpful?
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How should Product P7626, Phenylmethanesulfonyl fluoride (PMSF), solutions be prepared?
1 answer-
Stock solutions of 100-200 mM can be prepared in anhydrous isopropanol or anhydrous absolute ethanol. PMSF is unstable in the presence of water.
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