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C3640

Sigma-Aldrich

Carbonic Anhydrase Isozyme II from bovine erythrocytes

lyophilized powder, ≥2,000 W-A units/mg protein

Sinónimos:

Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II

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About This Item

Número de CAS:
9001-03-0
Comisión internacional de enzimas:
4.2.1.1 (BRENDA, IUBMB)
EC Number:
232-576-6
MDL number:
MFCD00081467
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

≥2,000 W-A units/mg protein

pI 

~5.4

storage temp.

−20°C

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Application

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme. The enzyme has also been used to generate CD4+ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications in pathologies such as glaucoma, epilepsy and Parkinson′s disease.

Unit Definition

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

inhibitor

Referencia del producto
Descripción
Precios

C4911

Chlorothiazide, thiazide diuretic

A6011

Acetazolamide, ≥99%, powder

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Todd S Davidson et al.
The American journal of pathology, 166(3), 729-736 (2005-03-04)
Autoimmune pancreatitis (AIP), a recently defined disease of unknown etiology, is characterized by inflammatory infiltrates in the pancreas with conspicuous involvement of the ducts. The disease clinically manifests in humans as epigastric pain, weight loss, and jaundice. This report describes
Daumantas Matulis et al.
Biochemistry, 44(13), 5258-5266 (2005-03-30)
ThermoFluor (a miniaturized high-throughput protein stability assay) was used to analyze the linkage between protein thermal stability and ligand binding. Equilibrium binding ligands increase protein thermal stability by an amount proportional to the concentration and affinity of the ligand. Binding
Wolfgang Maret
Advances in nutrition (Bethesda, Md.), 4(1), 82-91 (2013-01-16)
The nutritional essentiality of zinc for the growth of living organisms had been recognized long before zinc biochemistry began with the discovery of zinc in carbonic anhydrase in 1939. Painstaking analytical work then demonstrated the presence of zinc as a
Laurie C Hofmann et al.
Journal of experimental botany, 64(4), 899-908 (2013-01-15)
The concentration of CO(2) in global surface ocean waters is increasing due to rising atmospheric CO(2) emissions, resulting in lower pH and a lower saturation state of carbonate ions. Such changes in seawater chemistry are expected to impact calcification in
Romualdo Talento et al.
Ultrastructural pathology, 37(1), 70-76 (2013-02-07)
Despite progress in the classification of renal cell carcinomas (RCC), a subset of these carcinomas remains unclassified (RCC-U). Patients with RCC-U usually present at a late stage and have a poor prognosis. Several studies have attempted to extract new classifications
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Protocolos

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

Objective: To standardize a procedure for the enzymatic assay of Carbonic Anhydrase (EC 4.2.1.1) for Wilbur-Anderson Units.

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