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A9655

Sigma-Aldrich

Auramine O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Sinónimos:

4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

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About This Item

Fórmula empírica (notación de Hill):
C17H21N3 · HCl
Número de CAS:
2465-27-2
Peso molecular:
303.83
Colour Index Number:
41000
Beilstein/REAXYS Number:
4030061
EC Number:
219-567-2
MDL number:
MFCD00012484
UNSPSC Code:
12171500
PubChem Substance ID:
24891484
NACRES:
NA.47

product name

Auramine O, Dye content ≥80 %, certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, ≥80%

color

yellow to green

mp

>250 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL, yellow to orange

λmax

370 nm in H2O
432 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

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General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sigma-Aldrich

M9140

Methylene blue

Equilibrium and kinetic studies on sorption of basic dyes by a natural biopolymer poly (γ-glutamic acid).
Inbaraj B S, et al.
Biochemical Engineering Journal, 31(3), 204-215 (2006)
E W Koneman.
Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 1565-1565 (2006)
S Wilson et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 14(9), 1164-1168 (2010-09-08)
Direct sputum smear microscopy is usually less sensitive than the indirect approach using concentration by centrifugation, but laboratories often do not have access to appropriate equipment. An alternative method of sample concentration has been developed based on magnetic beads coated
Sumita Dixit et al.
Journal of AOAC International, 94(6), 1874-1881 (2012-02-11)
The present method utilizes a simple pretreatment step, cleanup on polyamide SPE cartridges, and HPLC resolution on reversed-phase C18 for the detection of the three basic nonpermitted dyes encountered in food matrixes. Polyamide cartridges were chosen because both acidic and
Nadav Amdursky et al.
The journal of physical chemistry. B, 116(45), 13389-13395 (2012-10-24)
There is an indispensable need for a fluorescence marker for the detection of amyloid fibrils, where, at present, the most used marker is thioflavin-T (ThT). Here, we present the use of auramine-O (AuO) as a possible alternative to ThT. As
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