A6410
Apirasa from potatoes
ATPase ≥200 units/mg protein, lyophilized powder
Sinónimos:
Adenosina 5′-difosfatasa, Adenosina 5′-trifosfatasa
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About This Item
Productos recomendados
tpo
Grade VI
Nivel de calidad
Formulario
lyophilized powder
ATPase activity
≥200 units/mg protein
secondary activity
≤15 % of base activity ADPase
mol peso
~49 kDa by gel filtration
composición
Protein, 30-60%
solubilidad
H2O: soluble 1.0 mg/mL
actividad extraña
Acid Phosphatase ≤2% of base activity
temp. de almacenamiento
−20°C
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Descripción general
Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.
Aplicación
Se encuentran al menos dos isoenzimas en diferentes variedades de S. tuberosum: una con una elevada relación ATPasa/ADPasa (∼10) y otra con una relación baja (∼1).
Reacción: ATP → ADP+Pi → AMP+2Pi.
Reacción: ATP → ADP+Pi → AMP+2Pi.
Apyrase from potatoes has been used:
- to deplete ATP
- to degrade endogenous extracellular ATP
- as a supplement in acid citrate dextrose solution to treat blood obtained from anesthetized mice
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
Acciones bioquímicas o fisiológicas
Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition. Apyrase has adenosine 5′-triphosphatase (ATPase) and adenosine 5′-diphosphatase (ADPase) activities.
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
Envase
Sold on the basis of ATPase units.
Otras notas
This grade has an exceptionally high ATPase/ADPase ratio.
Definición de unidad
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
Forma física
Partially purified, lyophilized powder containing potassium succinate buffer salts.
Nota de preparación
Derived from white potato
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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