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Merck

A6410

Sigma-Aldrich

Apirasa from potatoes

ATPase ≥200 units/mg protein, lyophilized powder

Sinónimos:

Adenosina 5′-difosfatasa, Adenosina 5′-trifosfatasa

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

tpo

Grade VI

Nivel de calidad

Formulario

lyophilized powder

ATPase activity

≥200 units/mg protein

secondary activity

≤15 % of base activity ADPase

mol peso

~49 kDa by gel filtration

composición

Protein, 30-60%

solubilidad

H2O: soluble 1.0 mg/mL

actividad extraña

Acid Phosphatase ≤2% of base activity

temp. de almacenamiento

−20°C

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Descripción general

Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.

Aplicación

Se encuentran al menos dos isoenzimas en diferentes variedades de S. tuberosum: una con una elevada relación ATPasa/ADPasa (∼10) y otra con una relación baja (∼1).
Reacción: ATP → ADP+Pi → AMP+2Pi.
Apyrase from potatoes has been used:
  • to deplete ATP
  • to degrade endogenous extracellular ATP
  • as a supplement in acid citrate dextrose solution to treat blood obtained from anesthetized mice

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .

Acciones bioquímicas o fisiológicas

Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition. Apyrase has adenosine 5′-triphosphatase (ATPase) and adenosine 5′-diphosphatase (ADPase) activities.
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Envase

Sold on the basis of ATPase units.

Otras notas

This grade has an exceptionally high ATPase/ADPase ratio.

Definición de unidad

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Forma física

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Nota de preparación

Derived from white potato

Pictogramas

Health hazard

Palabra de señalización

Danger

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Resp. Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Visite la Librería de documentos

Surfactant inhibits ATP-induced release of interleukin-1beta via nicotinic acetylcholine receptors
Backhaus S, et al.
Journal of Lipid Research, 58(6), 1055-1066 (2017)
Micromanipulation studies of chromatin fibers in Xenopus egg extracts reveal ATP-dependent chromatin assembly dynamics
Yan J, et al.
Molecular Biology of the Cell, 18(2), 464-474 (2007)
Stefan Golfier et al.
eLife, 9 (2020-05-13)
Loop extrusion by structural maintenance of chromosomes (SMC) complexes has been proposed as a mechanism to organize chromatin in interphase and metaphase. However, the requirements for chromatin organization in these cell cycle phases are different, and it is unknown whether
Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases1.
Smith T M & Kirley T L
Biochimica et Biophysica Acta, 1386(1), 65-78 (1998)
Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).
Nourizad N, et al.
Protein Expression and Purification, 27(2), 229-237 (2003)

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