A5691

Anti-actina, α-músculo liso monoclonal

clone 1A4, purified from hybridoma cell culture

• Sinónimos: SMA
• UNSPSC Code: 12352203
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 1A4, monoclonal
• form: buffered aqueous glycerol solution
• mol wt: antigen ~42 kDa
• species reactivity: human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
• technique(s): ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections; western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
• isotype: IgG2a
• UniProt accession no.: P62736
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Acta2(11475) ; rat ... Acta2(81633)

Descripción

General description

El anticuerpo (también conocido como anti-α-Sm-1) es específico para la isoforma α-actina de músculo liso. Reacciona de manera específica con la α-actina del músculo liso en análisis de inmunotransferencia y marca las células del músculo liso en cortes de tejido incluidas en parafina congeladas o fijadas con formalina.

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle).

Immunogen

Decapéptido N-terminal sintético de la α-actina de músculo liso.

Application

Se realizó inmunocitoquímica en células de músculo liso de aortas bovinas utilizando el anticuerpo monoclonal anti-ACTA2. Las células se cultivaron primero en cubreobjetos de vidrio y se fijaron en acetona al 50%/EtOH durante 10 minutos a 4 grados.

Se tiñeron inmunohistoquímicamente cortes incluidos en parafina de injertos de tejido testicular de rata con anticuerpos monoclonales anti-actina de músculo liso.
Se realizó análisis mediante IHC de tejido cardiaco de ratón teñido con x-gal utilizando el anticuerpo monoclonal primario anti-actina de músculo liso de ratón para identificar los miofibroblastos.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Immunocytochemistry was performed on kertocytes fixed in 1% PFA and incubated with mouse monoclonal anti-smooth muscle actin (14A) at a 1:100 dilution.

Mouse monoclonal anti-actin, α−smooth muscle-alkaline phosphatase antibody has been used for immunohistochemical application in mouse and human tissues.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide as a preservative.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)