This is not a species specific antigen, it is a slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. It has multi-species reactivity as it is a highly conserved region of the protein among species.
A5441
Anti-β-Actin (ACTB) Antibody
mouse monoclonal, AC-15
Sinónimos:
Anti-β-actina monoclonal
About This Item
Productos recomendados
Nombre del producto
Anti-β-actina monoclonal antibody produced in mouse, clone AC-15, ascites fluid
origen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
ascites fluid
tipo de anticuerpo
primary antibodies
clon
AC-15, monoclonal
mol peso
antigen 42 kDa
contiene
15 mM sodium azide
reactividad de especies
sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig
no debe reaccionar con
Dictyostelium discoideum
técnicas
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:1,000-1:2,000 using cultured human or chicken fibroblasts
western blot: 1:5,000-1:10,000 using cultured human or chicken fibroblast cell extracts
isotipo
IgG1
Nº de acceso UniProt
aplicaciones
research pathology
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)
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Descripción general
Especificidad
Inmunógeno
Aplicación
- en análisis de inmunofluorescencia
- en inmunotransferencia
- en inmunohistoquímica
- como control para micromatrices de proteínas
Acciones bioquímicas o fisiológicas
Forma física
Otras notas
Cláusula de descargo de responsabilidad
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Opcional
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
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Artículos
Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.
Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.
Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.
Actin isoforms are muscle tissue markers; six isoforms expressed, four for muscle differentiation, two ubiquitous.
Protocolos
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.
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species of antigen
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What IgG light chain (kappa or lambda) is associated with Clone AC-15 for Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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We have not determined the light chain used by monoclonal antibody AC-15. 95% of mouse antibodies have kappa light chains.
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What peroxidase-conjugated secondary antibody should I use with Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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We recommend using Product No. A9044, rabbit anti-mouse IgG, peroxidase conjugate as a secondary antibody. We also offer this same antibody directly conjugated to peroxidase as Product No. A3854.
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Can I use Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse, to detect mouse beta actin?
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This antibody will detect mouse beta actin. When used for western blotting, the secondary antibody may react with mouse IgG from the sample resulting with a bands at 50 kDa and 25 kDa (reducing conditions) or approximately 150 kDa (non-reducing conditions). It is best to use a directly conjugated antibody for immunohistochemistry or immunofluorescent assays. We offer Product numbers A3854, peroxidase conjugated.
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What is the molecular weight of Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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Beta Actin has a molecular weight of 42 kDa.
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Why do I have 3 bands on my western blot when using Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well. Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93:2234-2238, (1996).
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What is the antibody concentration of Product A5441, Monoclonal Anti-β-Actin antibody produced in mouse?
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This antibody is ascities fluid. The amount of protein present and the amount of IgG1 present is indicated on the Lot-specific Certificate of Analysis.
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