94740
Vanadyl ribonucleoside complexes
BioReagent, for molecular biology
Sinónimos:
Ribonucleoside vanadyl complexes, VRC
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About This Item
Productos recomendados
grade
for molecular biology
Quality Level
product line
BioReagent
form
powder
suitability
in accordance for ribonuclease inhibition test
shipped in
wet ice
storage temp.
−20°C
General description
Vanadyl ribonucleoside complexes are low molecular weight inhibitors of ribonucleases.
Application
Vanadyl ribonucleoside complexes (VDR) are transition state analogs that bind to active sites of many RNases, inhibiting their activity. Because the RNases are not covalently modified by the complexes, VDR must be used at every stage of RNA extraction and purification. However, VDR also inhibit RNA polymerases and in vitro translation and therefore must be removed from final preparation of RNA.
Vanadyl ribonucleoside complexes has been used in hybridization mixture for RNA in situ hybridization.
Biochem/physiol Actions
The vanadyl ribonucleoside complex has the ability to block the formation of ribosomal subunit in Staphylococcus aureus. The complex exhibits anti-microbial activity These complexes also serve as powerful inhibitors of several enzymes with ribonucleolytic activity.
related product
Referencia del producto
Descripción
Precios
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Effect of ribonucleoside-vanadyl complexes on enzyme-catalyzed reactions central to recombinant DNA technology
Biochemistry, 21(19), 4602-4608 (1982)
Analysis of TMEM174 gene expression in various renal cancer types by RNA in situ hybridization
Oncology Letters, 8(4), 1693-1696 (2014)
Isolation of cytoplasmic RNA: ribonucleoside-vanadyl complexes.
Methods in enzymology, 152, 227-234 (1987-01-01)
Inhibitors of RNases
Molecular Cloning: A Laboratory Manual, 1, 7-7 (2001)
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