30970

Sodium deoxycholate

BioXtra, ≥98.0% (dry matter, NT)

• Sinónimos: 3α,12α-Dihydroxy-5β-cholanic acid sodium salt, 7-Deoxycholic acid sodium salt, Desoxycholic acid sodium salt
• Fórmula empírica (notación de Hill): C₂₄H₃₉NaO₄
• Número de CAS: 302-95-4
• Peso molecular: 414.55
• Beilstein: 3581950
• Número CE: 206-132-7
• Número MDL: MFCD00064139
• Código UNSPSC: 12161900
• ID de la sustancia en PubChem: 57648497
• NACRES: NA.21

Propiedades

• origen biológico: ox bile
• Nivel de calidad: 300
• descripción: anionic
• Línea del producto: BioXtra
• Ensayo: ≥98.0% (dry matter, NT)
• Formulario: powder
• actividad óptica: [α]20/D +44±2°, c = 2% in H₂O
• mol peso: micellar avg mol wt 1200-5000
• número de agregación: 3-12
• técnicas: DNA purification: suitable; RNA purification: suitable; electrophoresis: suitable
• impurezas: ≤1% cholic acid (HPLC); ≤6% water
• CMC: 2-6 mM (20-25°C)
• solubilidad: water: soluble 1 gm in 10 ml, clear, colorless to very faintly yellow
• trazas de catión: Ca: ≤500 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤10 mg/kg; Mg: ≤10 mg/kg; Mn: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤10 mg/kg; Zn: ≤5 mg/kg
• HLB: 16
• grupo funcional: carboxylic acid
• cadena SMILES: [Na+].[H][C@]12CC[C@@]3([H])[C@]4([H])CC[C@H]([C@H](C)CCC([O-])=O)[C@@]4(C)[C@@H](O)C[C@]3([H])[C@@]1(C)CC[C@@H](O)C2
• InChI: 1S/C24H40O4.Na/c1-14(4-9-22(27)28)18-7-8-19-17-6-5-15-12-16(25)10-11-23(15,2)20(17)13-21(26)24(18,19)3;/h14-21,25-26H,4-13H2,1-3H3,(H,27,28);/q;+1/p-1/t14-,15-,16-,17+,18-,19+,20+,21+,23+,24-;/m1./s1
• Clave InChI: FHHPUSMSKHSNKW-SMOYURAASA-M

Descripción

Descripción general

Sodium Deoxycholate, a bile acid derivative, is a crucial component in biochemical and cell biology research. This water-soluble detergent is widely used in protein-related experiments, including cell lysates and liposome preparation. It aids in membrane protein extraction, lipid compound extraction, and cell nucleus separation. Sodium Deoxycholate is also applied in cholesterol flocculation tests and affinity chromatography for column elution or regeneration. It is commonly used in microbiology for cell lysing, especially because it can be removed from the solution via dialysis.

Sodium Deoxycholate is also utilized in eluting buffers for affinity chromatography and plays a crucial role in DNA and RNA extraction from cells and tissues in molecular biology applications. Additionally, it is used as a catalyst in the preparation of protein extracts for immunoblotting and immunoprecipitation of radiolabeled proteins.

Aplicación

Sodium deoxycholate has been used for preparation of protein extracts for immunoblotting and immunoprecipitation of radiolabeled proteins.

Acciones bioquímicas o fisiológicas

Sodium deoxycholate is a bile salt that stimulates the apical to basolateral accumulation of epirubicin in Caco-2 cells as well as increased serosal absorption of epirubicin in the rat jejunum and ileum. Sodium deoxycholate dissociates histones II from DNA as well as causes lysis of the viral membrane.

Características y beneficios

• Suitable for Cell Biology and Biochemical Research
• Can be used in Electrophoresis, DNA and RNA purification
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
• High purity product for research applications

Otras notas

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Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H302
• Consejos de prudencia: P264 - P270 - P301 + P312 - P501
• Clasificaciones de peligro: Acute Tox. 4 Oral
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves