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P1754

Sigma-Aldrich

TWEEN® 80

TWEEN® 80

viscous liquid

Sinónimos:

Monooleato de POE (20) sorbitano, Monooleato de polietilenglicol sorbitano, Monooleato de polioxietilen sorbitano, Polisorbato 80

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About This Item

Número de CAS:
9005-65-6
MDL number:
MFCD00082107
UNSPSC Code:
12161900
NACRES:
NA.21
En este momento no podemos mostrarle ni los precios ni la disponibilidad

biological source

Porcine (tallow)
bovine (tallow)

Quality Level

200

description

non-ionic

form

viscous liquid

mol wt

micellar avg mol wt 79,000
average mol wt 1310

composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

aggregation number

60

CMC

0.012 mM (20-25°C)

viscosity

400-620 mPa.s

transition temp

cloud point 65 °C

solubility

water: soluble, clear to slightly hazy, colorless to yellow

density

1.064 g/cm3

HLB

15

application(s)

cell analysis

SMILES string

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

InChI key

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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General description

Tween® 80 is a non-ionic detergent and surfactant, classified as an ester of sorbitan and oleic acid. It is a versatile compound commonly employed in various biological applications, including cell lysis, nuclei isolation, and cell fractionation. Additionally, Tween 80 finds use in stabilizing proteins and facilitating protein membrane studies. Its effectiveness stems from its membership in the polysorbate group, characterized by the combination of sorbitol and sorbitane oleic esters with polyethylene glycol chains. These structural features contribute to Tween 80′s emulsifying capabilities and a broad range of applications.

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
TWEEN® 80 has been used in drug vehicle solution for specific injections in mice and rats.[1][2] It has been used for the determination of esterase activity in bacterial cells.[3]

Biochem/physiol Actions

TWEEN® 80 is a polysorbate surfactant which helps in stabilizing proteins and in protein membrane studies. It is regularly used for the extraction of membrane proteins and isolation of nuclei from cells.[4][5][6]

Features and Benefits

  • Excellent wetting and dispersing agent
  • Highly versatile surfactant for your Cell Biology and Biochemical research

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC

comparable product

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

>235.4 °F - closed cup

flash_point_c

> 113 °C - closed cup

ppe

Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
BCCM7843
BCCM6321
BCCM3263
BCCL4939
BCCL8221

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Questions

1–10 of 11 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. This material is free of residual solvents?

    1 answer

    1. This product is not tested for residual solvents. Should this be a requirement, see product 817061, which is tested for Ethylene Oxide, 1,4-Dioxane, and Ethylene glycol. Please see the link below to review this item:
      https://www.sigmaaldrich.com/product/mm/817061

      Helpful?

  4. What is the boiling point of this specific Tween 80 product?

    1 answer

    1. This product is not tested for boiling point. However, various reference sources cite a boiling point of approximately 100°C.

      Helpful?

  5. Hello, Is there an expiration date or retest date for this product?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 4 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  6. What is the Critical Micelle Concentration (CMC) of Product P1754, TWEEN® 80?

    1 answer

    1. The CMC value is 13-15 mg/Liter.

      Helpful?

  7. Is Product P1754, TWEEN® 80, tested for RNase and DNase activity?

    1 answer

    1. No. Product P1754 is not tested for contaminating RNase and DNase activity.  Product P5188 is a Molecular Biology grade product that has been tested for DNase and RNase.

      Helpful?

  8. Is Product P1754, TWEEN® 80, cell culture tested?

    1 answer

    1. No, P1754 is not cell culture tested, but product P4780 is cell culture tested TWEEN 80.

      Helpful?

  9. Is it normal for the color of Product P1754, TWEEN® 80, to change from lot to lot?

    1 answer

    1. Yes. According to Sigma-Aldrich's specifications, Product No. P1754 can appear bright yellow to yellow with a green cast.  Please see our Specification Sheet for more information.

      Helpful?

  10. Is Product P1754, TWEEN® 80, sterile?

    1 answer

    1. The product is not sterile. Autoclaving a solution of TWEEN 80 is generally not advised. Sterile filtration is more easily done if the liquid is warmed to about 40°C and alternate portions of hot distilled water and TWEEN 80 are poured through the 0.22 μm filter. The TWEEN 80 will blend and remain in solution.

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1–10 of 11 Questions  

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