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11034731001

Roche

Primer "random"

lyophilized, sufficient for ≤400 reactions, pkg of 2 mg (Primer, Random pd(N)6 Potassium Salt)

Sinónimos:

primer

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About This Item

UNSPSC Code:
41106300

form

lyophilized

Quality Level

100

usage

sufficient for ≤400 reactions

packaging

pkg of 2 mg (Primer, Random pd(N)6 Potassium Salt)

manufacturer/tradename

Roche

storage temp.

−20°C

Categorías relacionadas

General description

p(dN)6

Application

Primer "random" has been used:
  • in a reverse transcription-polymerase chain reaction (RT-PCR) and quantitative PCR (qPCR) to retro-transcribe RNA
  • to reverse-transcribe RNA samples to cDNA
  • in digital droplet PCR (ddPCR) to synthesis cDNA from RNA

Primer for cDNA synthesis

Features and Benefits

  • Offers a consistent representation of all RNA sequences in mRNA
  • Can prime cDNA transcription from RNAs lacking a poly(A) tail
  • Short cDNA transcripts can overcome the challenges posed by RNA secondary structures
  • The average length of cDNAs formed can be controlled by adjusting the ratio of random primers to RNA in the reverse transcription (RT) reaction

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

does not flash

flash_point_c

does not flash

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Amaresh C Panda et al.
Nucleic acids research, 44(5), 2393-2408 (2016-01-29)
Skeletal muscle contains long multinucleated and contractile structures known as muscle fibers, which arise from the fusion of myoblasts into multinucleated myotubes during myogenesis. The myogenic regulatory factor (MRF) MYF5 is the earliest to be expressed during myogenesis and functions
Vadim Alexandrov et al.
Nature biotechnology, 34(8), 838-844 (2016-07-05)
Rapid technological advances for the frequent monitoring of health parameters have raised the intriguing possibility that an individual's genotype could be predicted from phenotypic data alone. Here we used a machine learning approach to analyze the phenotypic effects of polymorphic
Monika Hiller et al.
PloS one, 13(10), e0204485-e0204485 (2018-10-03)
Duchenne muscular dystrophy is a lethal disease caused by lack of dystrophin. Skipping of exons adjacent to out-of-frame deletions has proven to restore dystrophin expression in Duchenne patients. Exon 51 has been the most studied target in both preclinical and
Vittoria Pagliarini et al.
Journal of neurochemistry, 153(2), 264-275 (2019-12-08)
Spinal muscular atrophy (SMA) is a motor neuron disease caused by loss of function mutations in the Survival Motor Neuron 1 (SMN1) gene and reduced expression of the SMN protein, leading to spinal motor neuron death, muscle weakness and atrophy.
Tae-Dong Kim et al.
The Journal of clinical investigation, 126(2), 706-720 (2016-01-06)
Histone demethylase upregulation has been observed in human cancers, yet it is unknown whether this is a bystander event or a driver of tumorigenesis. We found that overexpression of lysine-specific demethylase 4A (KDM4A, also known as JMJD2A) was positively correlated
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