Name: Custom qPCR Probes
Brand: SIGMA

Product Resources Title

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<h2>Dual-Labeled Probes</h2>


<ul>
<li>Amounts: 1, 5, and 5 OD</li>
<li>Purification: HPLC</li>
<li>Sequence Lengths: 30 - 60 bases</li>
<li>Modifications: Locked Nucleic Acid available</li>
<li>Dyes: Comprehensive offering of reporters and quenchers</li>
<li>Quality Control: 100% mass spectrometry</li>
<li>Format: Supplied dry in amber tubes</li>
<li><a href="/technical-documents/technical-article/genomics/qpcr/scorpions-probes">Learn more</a></li>
</ul>


<h2>Black Hole Quencher™ (BHQ™)</h2>


<ul>
<li>Amounts: 1, 3, 5, and 10 OD</li>
<li>Purification: HPLC</li>
<li>Sequence Lengths: 15 - 40 bases</li>
<li>Modifications: Locked Nucleic Acid available</li>
<li>Dyes: Comprehensive offering of reporters and quenchers</li>
<li>Quality Control: 100% mass spectrometry</li>
<li>Format: Supplied dry in amber tubes</li>
<li><a href="/technical-documents/technical-article/genomics/qpcr/molecular-beacons">Learn more</a></li>
</ul>


<ul>
<li>Available for all Custom qPCR Probe Types</li>
<li>Increased thermal stability and hybridization specificity</li>
<li>Easier and more specific designs for problematic target sequences</li>
<li><a href="/technical-documents/technical-article/genomics/pcr/locked-nucleic-acids-faq">Learn more</a></li>
</ul>


<ul>
<li>Available for all Custom qPCR Probe types</li>
<li>No native fluorescence (emits heat instead of light), resulting in lower background fluorescence</li>
<li>Increased signal-to-noise ratio, providing higher sensitivity</li>
<li>Enables a wider choice of reporters for multiplexing</li>
<li><a href="/technical-documents/technical-article/genomics/qpcr/black-hole-quenchers">Learn more</a></li>
</ul>


<ul>
<li>Amounts: 1, 3, 5, and 10 OD</li>
<li>Purification: HPLC</li>
<li>Sequence Lengths: 15 - 40 bases</li>
<li>Modifications: Locked Nucleic Acid available</li>
<li>Dyes: Comprehensive offering of reporters and quenchers</li>
<li>Quality Control: 100% mass spectrometry</li>
<li>Format: Supplied dry in amber tubes</li>
<li><a href="/technical-documents/technical-article/genomics/qpcr/dual-labeled-probes">Learn more</a></li>
</ul>


<ul>
<li>Amounts: 0.1, 0.25, 1.5, and 15 OD</li>
<li>Purification: HPLC</li>
<li>Sequence Lengths: 15 - 40 bases</li>
<li>Modifications: Locked Nucleic Acid available</li>
<li>Dyes: Probe 1 fluorescein (3′ end); Probe 2 phosphate (3′ end) and LC Red 610, 640, 670 or 705 (5′ end)</li>
<li>Quality Control: 100% mass spectrometry</li>
<li>Format: Supplied dry in amber tubes</li>
<li><a href="/technical-documents/technical-article/genomics/qpcr/lightcycler-probes">Learn more</a></li>
</ul>


<h2>LightCycler<sup>®</sup> Probes</h2>


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Custom qPCR Probes Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR; Synonyms: BHQ,BHQ Probes,Black Hole Quencher,Locked Nucleic Acid probes,Probes,Real-Time PCR Probes,locked nucleic acid,qPCR assays,qPCR designs at Sigma-Aldrich

Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR

Custom qPCR Probes Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR

Key Documents

Custom qPCR Probes

Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR

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