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SCM075

Sigma-Aldrich

Inhibidor ROCK (Y-27632)

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

Sinónimos:

Y-27632

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Quality Level

100

form

liquid

mol wt

320.26 g/mol

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

General description

El inhibidor ROCK (Y-27632) es un inhibidor competitivo del ATP selectivo de la proteína cinasa asociada a Rho (ROCK) que incluye p160Rock (Ki=140 nM) y Rock-II También actúa como un potente inhibidor de la sensibilización al Ca2+ inducida por agonista de la fosforilación de miosina y la contracción del músculo liso. El tratamiento de una hora con Y-27632 10uM bloquea la apoptosis de las células madre embrionarias humanas (hESCs) cultivadas disociadas, aumenta la supervivencia y la eficacia de clonación de las hESCs sin afectar a su pluripotencia1. Recientemente algunos informes demostraron que la aplicación de Y-27632 y Accutase (Nº de ref. SCR005), una mezcla formulada de enzimas digestivas, mejora la tasa de supervivencia pos-descongelamiento de las hESC individuales utilizando métodos convencionales de congelación lenta y descongelación rápida

Storage and Stability

Antes de la reconstitución: Mantener a -20ºC, protegido de la luz durante 2 años. Después de la reconstitución: 1 años a -20ºC, protegido de la luz

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Malgorzata Bienkowska-Haba et al.
Journal of virology, 94(3) (2019-11-22)
It is established that the host cell transcriptomes of natural lesions, organotypic rafts, and human papillomavirus (HPV)-immortalized keratinocytes are altered in the presence of HPV genomes. However, the establishment of HPV-harboring cell lines requires selection and immortalization, which makes it
Maike Frye et al.
eLife, 9 (2020-09-09)
Endothelial integrity is vital for homeostasis and adjusted to tissue demands. Although fluid uptake by lymphatic capillaries is a critical attribute of the lymphatic vasculature, the barrier function of collecting lymphatic vessels is also important by ensuring efficient fluid drainage
Ambra Gianotti et al.
Frontiers in pharmacology, 9, 1176-1176 (2018-11-13)
Cystic fibrosis (CF) is a chronic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, which encodes for a channel expressed at the apical surface of epithelial tissues. Defective chloride and bicarbonate secretion, arising from CFTR mutations
Marta Słoniecka et al.
Journal of molecular medicine (Berlin, Germany), 97(10), 1477-1489 (2019-08-11)
Fibrosis is characterized by hardening, overgrowth, and development of scars in various tissues as a result of faulty reparative processes, diseases, or chronic inflammation. During the fibrotic process in the corneal stroma of the eye, the resident cells called keratocytes
Anushree C Gulvady et al.
Molecular biology of the cell, 30(11), 1298-1313 (2019-03-21)
Fibroblasts transformed by the proto-oncogene Src form individual invadopodia that can spontaneously self-organize into large matrix-degrading superstructures called rosettes. However, the mechanisms by which the invadopodia can spatiotemporally reorganize their architecture is not well understood. Here, we show that Hic-5
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