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Key Documents

MABC1131

Sigma-Aldrich

Anti-PD-L1 B7-H1 Antibody, clone 10B5

clone 10B5, from hamster(Armenian)

Sinónimos:

Programmed cell death 1 ligand 1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

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biological source

hamster (Armenian)

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10B5, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable (paraffin)

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

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Este artículo
MABF404MABC1115MABC1132
species reactivity

mouse

species reactivity

mouse

species reactivity

human

species reactivity

mouse

clone

10B5, monoclonal

clone

10F.9G2, monoclonal

clone

5H1, monoclonal

clone

G4, monoclonal

biological source

hamster (Armenian)

biological source

rat

biological source

mouse

biological source

hamster (Armenian)

Gene Information

mouse ... Cd274(60533)

Gene Information

mouse ... Cd274(60533)

Gene Information

human ... CD274(29126)

Gene Information

mouse ... Pdcd1(18566)

UniProt accession no.

Q9EP73

UniProt accession no.

Q9EP73

UniProt accession no.

Q9NZQ7

UniProt accession no.

Q02242

General description

Programmed cell death 1 ligand 1 (UniProt: Q9EP73; also known as PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274) is encoded by the Cd274 (also known as B7h1, Pdcd1l1, Pdcd1lg1, Pdl1) gene (Gene ID: 50533) in murine species. PD-L1 is a single-pass type I membrane protein that is highly expressed in the heart, thymus, skeletal muscle, and lung. Its expression is also reported on activated dendritic cells, B-cells, and macrophages. Numerous tumor cell lines of lymphoid origin also express PD-L1. PD-L1 expression can be up-regulated by interferon-gamma treatment in monocytes, B cells, and macrophages. PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained. However, tumors and pathogens that cause chronic infections can exploit this pathway to escape T-cell mediated tumor-specific and pathogen-specific immunity. The effector functions of T-cells expressing PD-1 can be downregulated by PD-L1 or PD-L2 expressed by the tumor cells. PD-L1 is initially produced with signal peptide (aa. 1-18) sequence, the removal of which yields the mature protein with a large extracellular (aa. 19-239) domain, a transmembrane domain (aa 240-260), and a cytoplasmic domain (aa 261-290).

Specificity

This Armenian hamster monoclonal antibody detects Programmed cell death 1 ligand 1 (PD-L1) in murine species.

Immunogen

Epitope: extracellular domain
Recombinant fragment corresponding to the extracellular domain of Mouse Programmed cell death 1 ligand 1.

Application

Anti-PD-L1 B7-H1, clone 10B5, Cat. No. MABC1131, is an Armenian hamster monoclonal antibody that detects Programmed cell death 1 ligand 1 (PD-L1) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Inhibition Function assay.
Inhibits Activity/Function Analysis: A representative lot blocked the activity of PD-L1 B7-H1 and inhibited apoptosis of activated alloreactive 2C T cells. (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Goldberg, M.V., et. al. (2007). Blood. 110(1):186-92; Joshi, T., et. al. (2009). PLoS Pathog. 5(5):e1000431; Liu, X., et. al. (2016). Sci Rep. 6:36722).



Flow Cytometry Analysis: A representative lot detected PD-L1 B7-H1 in Flow Cytometry applications (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Dong, H., et. al. (2004). Immunity. 20(3):327-36).
Research Category
Apoptosis & Cancer

Quality

Evaluated by Immunohistochemistry (Paraffin) in mouse heart tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected PD-L1 B7-H1 in mouse heart tissue sections.

Target description

32.78 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified armenian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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