524629

Phosphatase Inhibitor Cocktail V

50X, liquid, for the inhibition of serine/threonine and tyrosine phosphatase

• Sinónimos: Phosphatase Inhibitor Mix Set V, Phosphatase inhibitor
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Nombre del producto: Phosphatase Inhibitor Cocktail Set V, 50X, Phosphatase Inhibitor Cocktail Set V is a 50X cocktail of four serine/threonine and tyrosine phosphatase inhibitors.
• Formulario: liquid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• solubilidad: water: soluble
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

A cocktail of four phosphatase inhibitors for the inhibition of both serine/threonine and protein tyrosine phosphatases. Available as 1 ml or as a set of five 1 ml vials.

Aplicación

Phosphatase Inhibitor Cocktail Set V, 50X has been used:

• as a supplement in the lysis buffer to dissociate metabolically activated one (MMe) bone marrow macrophages (BMDMs) for Western blot
• in radio-immunoprecipitation assay (RIPA) modified buffer to incubate cultured human umbilical vein endothelial cells (HUVECs) for western blot
• in lysis buffer to lyse cultured HUVECs for immunoprecipitation
• in wash buffer to wash the protein complexes
• as a component in 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer to homogenize J774 cells to assess the effect of lipopolysaccharides (LPS) incubation on cytosolic phospholipase A2 (cPLA2) activity

Acciones bioquímicas o fisiológicas

Protein phosphorylation and dephosphorylation play a crucial role in signal transduction, controlling various cellular processes such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific serine, threonine, or tyrosine residues, which alters the protein′s conformation and its ability to interact with ligands. Protein phosphatases then remove the phosphate group, restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is of utmost importance to preserve their phosphorylation state.

Características y beneficios

• Convenient: formulations are ready-to-use, making them easy to use.
• Consistent: high-quality formulations ensure reliable and reproducible performance.
• Flexible: the cocktails are designed to target different categories of phosphatases, offering versatility in experimentation.

Componentes

Each vial contains 1 ml of aqueous solution with the following components:

• 250 mM Sodium Fluoride
• 50 mM ß-Glycerophosphate (Cat. No. 35675)
• 50 mM Sodium Pyrophosphate Decahydrate
• 50 mM Sodium Orthovanadate      

Advertencia

Toxicity: Toxic (F)

Forma física

In H₂O.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C) for long-term storage. Dilute 1:50 just prior to use.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H319
• Consejos de prudencia: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Clasificaciones de peligro: Eye Irrit. 2
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable