524627
Phosphatase Inhibitor Cocktail III
liquid, for the inhibition of serine/threonine and protein tyrosine phosphatases
Sinónimos:
Phosphatase Inhibitor Mix (Set III), Phosphatase inhibitor
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.77
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Nombre del producto
Phosphatase Inhibitor Cocktail Set III, Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.
Formulario
liquid
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
solubilidad
water: soluble
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Descripción general
Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.
Aplicación
Phosphatase Inhibitor Cocktail Set III has been used:
- as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
- to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
- as a component in cell lysis buffer to extract total proteins for western blotting
Acciones bioquímicas o fisiológicas
Cell permeable: no
Primary Target
Serine/threonine and protein tyrosine phosphatases
Serine/threonine and protein tyrosine phosphatases
Product does not compete with ATP.
Reversible: no
Características y beneficios
- Ready-to-use formulations for convenient use
- High-quality products for consistent and reproducible performance,
- Flexible options with a range of cocktails targeting different categories of phosphatases
Componentes
Each vial contains 1 ml of aqueous solution with the following components:
- 50 mM Sodium Fluoride
- 10 mM ß-Glycerophosphate (Cat. No. 35675)
- 10 mM Sodium Pyrophosphate Decahydrate
- 1 mM Sodium Orthovanadate
Advertencia
Toxicity: Toxic (F)
Forma física
In H₂O.
Reconstitución
For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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