218800
Caspase-7, Human, Recombinant, E. coli
Caspase-7, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.
Sinónimos:
Mch3, ICE-LAP3
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About This Item
UNSPSC Code:
12352202
Productos recomendados
recombinant
expressed in E. coli
Quality Level
form
lyophilized
specific activity
≥15,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−70°C
General description
M.W. 12,000 and 20,000 (heterodimer).
Recombinant, human caspase-7 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. Useful for the study of enzyme regulation and kinetics, cleavage of target substrates, and inhibitor screening. MW: 12000 and 20000.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will cleave 1 nmol of Ac-DEVD-pNA (Cat. No. 235400) per hour at 37°C, pH 7.2.
Reconstitution
Following reconstitution, aliquot and freeze (-70°C). Stock solitions are stable for up to 3 months at -70°C.
Reconstitute to 1 unit/µl in PBS, 15% glycerol.
Other Notes
Stennicke, H.R., and Salvesen, G.S. 1997. J. Biol. Chem.272, 25719.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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