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C1224

Sigma-Aldrich

Caspase 3 human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein

Sinónimos:

Apopain, CPP32, Yama

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About This Item

Comisión internacional de enzimas:
3.4.22.56 (BRENDA, IUBMB)
MDL number:
MFCD01634593
UNSPSC Code:
12352204
NACRES:
NA.32

recombinant

expressed in E. coli (C-terminal histidine-tagged)

Quality Level

200

assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥1.0 units/mg protein

mol wt

~30 kDa

UniProt accession no.

P42574

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CASP3(836)

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General description

Research area: Apoptosis

Human recombinant C-terminal histidine tagged caspase 3 is a fully active protein consisting of 17 kDa and 13.5 kDa subunits; the 13.5 kDa subunit contains the histidine tag.

Application

Caspase 3 human has been used to digest human r-vimentin. It has also been used in caspase-3 inhibition assay and protease inhibition assay.

This product may also be used in cell-based apoptosis assay.

Biochem/physiol Actions

Caspase 3 is a member of the CED-3 subfamily of caspases and is responsible for the cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP), the inhibitor of caspase-activated deoxyribonuclease (ICAD), and gelsolin, a protein involved in apoptosis regulation. Caspase 3 is considered to be an effector caspase, activating pro-caspase 6 and pro-caspase 9 in vitro. Caspase 3 can be activated by caspase 8, caspase 6, and granzyme B.

Unit Definition

One unit will cleave 1.0 μmole of N-acetyl-Asp-Glu-Val-Asp-pNA per min at pH 7.4 at 25 °C.

Physical form

Solution in 10% (w/v) glycerol containing 50 mM HEPES, pH 7.4, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS

Preparation Note

Caspase 3 is synthesized as a 32 kDa proenzyme. The active enzyme is a heterodimer of two large (17 kDa) subunits and two small (12 kDa) subunits.

Inhibitor

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Descripción
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Substrate

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antibody

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related product

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Descripción
Precios

CASP3F

Caspase 3 Assay Kit, Fluorimetric

CASP3C

Caspase 3 Assay Kit, Colorimetric

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Hight MR, et al.
Clinical Cancer Research, 20(8), 2126-2135 (2014)
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