Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

1.12018

Sigma-Aldrich

Albumin fraction V

(from bovine serum) for biochemistry

Sinónimos:

Albumin fraction V, Bovine serum albumin, Serum albumin

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Número de CAS:
90604-29-8
UNSPSC Code:
12352202
EC Index Number:
292-322-5
NACRES:
NA.21

form

solid

pH

6.8-7.2 (20 °C, 1 g/L in H2O)

solubility

1 g/L

bulk density

150 kg/m3

storage temp.

2-8°C

Categorías relacionadas

General description

Bovine serum albumin (BSA) belongs to the serum albumin family with three domains and two sub-domains each. This globular, α-helical, non-glycosylated protein has 17-disulfide bonds.

Application

Albumin fraction V has been used:
  • as a component of perfusion buffer for perfusion protocol for rodent organ perfusion
  • as a blocking agent in western blot analysis
  • as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays

Biochem/physiol Actions

Bovine Serum Albumin (BSA) is the chief component of cell culture media and also works as a blocking agent in enzyme-linked immunosorbent assay (ELISA). It is beneficial for embryonic stem cells (hESC) differentiation and assists the transport of drugs, hormones, and fatty acids. 

Analysis Note

Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Thomas Franze et al.
The Analyst, 129(7), 589-596 (2004-06-24)
Three monoclonal antibodies (mAb) and three polyclonal antibodies (pAb) have been characterized and compared with respect to their cross-reactivities and affinities for 3-nitrotyrosine, eight aromatic compounds with similar chemical structures, a peptide containing a single nitrotyrosine residue, and fourteen nitrated
Sebastian Ivens et al.
Brain : a journal of neurology, 130(Pt 2), 535-547 (2006-11-24)
It has long been recognized that insults to the cerebral cortex, such as trauma, ischaemia or infections, may result in the development of epilepsy, one of the most common neurological disorders. Human and animal studies have suggested that perturbations in
J L Lanciego et al.
Brain research. Brain research protocols, 2(4), 323-332 (1998-06-19)
The present report deals with a multiple tract-tracing procedure in non-human primates enabling the simultaneous visualization of retrogradely transported Fluoro-Gold (FG) and cholera toxin B subunit (CTB) in combination with anterogradely transported biotinylated dextran amine (BDA). Two issues have played
Holger Jungbluth et al.
Clinical oral investigations, 18(9), 2077-2086 (2014-02-08)
The aim of this series of studies was the development and validation of a new model for evaluation of dentinal hypersensitivity (DH) therapies. Roots from extracted human teeth were sealed with a flowable composite. In the cervical area, a 3-mm-wide
Charlotte Bayer Christiansen et al.
American journal of physiology. Gastrointestinal and liver physiology, 315(1), G53-G65 (2018-03-02)
The colonic epithelium harbors a large number of endocrine cells, but little is known about the endocrine functions of the colon. However, the high density of glucagon like peptide-1 (GLP-1)- and peptide-YY (PYY)-secreting L cells is of great interest because
Ver todos los artículos relacionados

Artículos

Determination of Protein by Bicinchoninic Acid Assay

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

Determination of Protein by Bicinchoninic Acid Assay

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

Determination of Protein by Bicinchoninic Acid Assay

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

Determination of Protein by Bicinchoninic Acid Assay

BCA protein assay or Smith assay to determine protein concentration in a sample using Spectroquant® Prove photometers.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico