LC/MS/MS Analysis of Estradiol (Underivatized) from Patient Serum on Titan C18, 10 cm Column
CONDITIONS
column
Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)
mobile phase
[A] 0.2 mM ammonium fluoride; [B] methanol
gradient
40 to 82%B in 4.6 min; to 100%B in 0.05 min; held for 1.15 min; to 40% B in 0.05 min; held for 1.65 min
flow rate
0.4 mL/min
column temp.
40 °C
detector
MS, ESI(-), MRM transitions listed on figure
injection
20 μL
sample
patient serum sample containing estradiol, 56 pmol/L
说明
分析報告
A fast and robust LC/MS/MS method was developed for the measurement of estradiol in human serum using Titan C18 1.9 μm columns. Ultra high purity LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant certified reference material grade standards provided reliable quantification.
法律資訊
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