HPLC Analysis of Metabolites of 7,12-Dimethylbenz[a]anthracene on SUPELCOSIL^™ LC-18

column

SUPELCOSIL LC-18, 25 cm × 4.6 mm I.D., 5 μm particles (58298)

mobile phase

[A] methanol:[B] water, (50:50, A:B), 10 min to 100:0 at 2.5% /min

flow rate

0.8 mL/min

detector

fluorescence

sample

rat liver, 9000 × g supernatant fraction from Aroclor-treated animals