HPLC Analysis of Luciferin Enantiomers on Astec® CHIROBIOTIC® V
CONDITIONS
column
CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase
[A] 20 mM ammonium nitrate, pH 5.5; [B] THF; (90:10, A:B)
flow rate
1 mL/min
column temp.
ambient
detector
UV, 254 nm
injection
6 μL
sample
0.5 mg/mL in methanol
说明
法律資訊
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC