HPLC Analysis of Tryptic Digest on BIOshell A160 Peptide C18

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 0.1% formic acid in water; [B] 25:75, (0.4% formic acid in water):acetonitrile

gradient

0 to 70% B in 40 min.

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI(+), TOF

injection

2 μL

sample

10 pg/L in 0.1% formic acid