HPLC Analysis of Proteins on BIOshell A160 Peptide C18

材料

分析柱

产品编号

说明

价格

53307-U

Ascentis^® Express Peptide ES-C18, 2.7μm HPLC 色谱柱

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

標準

产品编号

说明

价格

T9128

胰蛋白酶抑制剂来源于大豆

lyophilized powder

A2327

Adrenomedullin 52 human

≥90% (HPLC), powder

R6513

核糖核酸酶A 来源于牛胰腺

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

62971

Lysozyme 来源于鸡蛋白

powder (crystalline), 70000-140000 U/mg

S7571

超氧化物歧化酶来源于牛红细胞

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

41235

卵清蛋白-Atto 488 偶联物

suitable for fluorescence

C6749

细胞色素 C 马

recombinant, expressed in E. coli

C2624

碳酸酐酶来源于牛红细胞

≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile

gradient

held at 0% B for 0.5 min; 0 to 100% B in 7 min

flow rate

0.3 mL/min

pressure

4743 psi (327 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

1 μL

sample

0.2 - 0.6 g/L in mobile phase A

说明

法律資訊

null

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