HPLC Analysis of Four Proteins on Zenix® SEC-300, Comparison of Column Internal Diameter
材料
分析色谱柱
Zenix® SEC-300 Gel Filtration Column, 3 μm
L × I.D. 30 cm × 2.1 mm, 3 μm particle size, stainless steelZenix® SEC-300 Gel Filtration Column, 3 μm
L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steelZenix® SEC-300 Gel Filtration Column, 3 μm
L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steel标准
CONDITIONS
column
Zenix SEC-300, 30 cm x 2.1 mm I.D., 3 μm particles (Z777024)
column
Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)
column
Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)
mobile phase
0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid
flow rate
15, 72, 207 μL/min (2.1 mm ID, 4.6 mm ID, 7.8 mm ID, respectively)
pressure
220, 240, 350 psi (2.1 mm ID, 4.6 mm ID, 7.8 mm ID, respectively)
column temp.
25 °C
detector
UV, 215 nm
injection
0.3, 1.4, 4.0 μL (2.1 mm ID, 4.6 mm ID, 7.8 mm ID, respectively)
sample
(Waters SEC200 Protein Std)
说明
分析说明
Smaller column internal diameters (ID) are attractive for reasons of low solvent consumption. However, when adequate sample is available, larger ID analytical columns can be advantageous because they provide better column performance.
法律信息
Zenix is a registered trademark of Sepax Technologies