HPLC Analysis of Dorzolamide Enantiomers on Astec^® CHIROBIOTIC^® V2

column

CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)

injection

10 μL

mobile phase

[A] 3.81 mM ammonium trifluoroacetic acid; [B] methanol; (5:95, A:B)

flow rate

0.3 mL/min

column temp.

22 °C

detector

UV, 254 nm

sample

1 mg/mL in methanol