HPLC Analysis of Cyclophosphamide Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] ethanol; [C] THF; (90:5:5, A:B:C)

flow rate

0.75 mL/min

column temp.

23 °C

detector

UV, 205 nm

injection

5 μL

sample

1 mg/mL in methanol