HPLC Analysis of Anthocyanins in Red Wine on Ascentis^® C18

column

Ascentis C18, 25 cm × 4.6 mm I.D., 5 μm particles (581325-U)

mobile phase

[A] water:formic acid (9:1), [B] acetonitrile:water:formic acid (5:4:1)

flow rate

1 mL/min

column temp.

25 °C

detector

VIS, 518 nm

injection

10 μL

sample

red wine diluted with water, centrifuged and passed through a 0.45 μm filter