HPLC Analysis of 7-Azatryptophan Enantiomers on Astec® CHIROBIOTIC® T
CONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] water; [B] ethanol; (70:30, A:B)
flow rate
0.8 mL/min
column temp.
ambient
detector
UV, 210 nm
injection
5 μL
sample
5 mg/mL in methanol/water/ammonium hydroxide
说明
法律資訊
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC