GC Analysis of Amphetamines in Urine on a 100% Methyl Column after SPME using 100 μm PDMS Fiber
材料
SPME 光纤
产品编号
说明
价格
SPME 纤维组件聚二甲基硅氧烷 (PDMS)
100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, plain red hubCONDITIONS
sample/matrix
1 mL urine + 0.7g potassium carbonate in 20 mL headspace vial, equilibrated at 80°C, 30 min
SPME fiber
polydimethylsiloxane, 100 μm (57300-U)
derivatization
methyl bis-trifluoroacetamide (headspace, 0.5 min, ambient)
extraction
headspace, 3-5 min, 80 °C
desorption process
1 min, 270 °C
column
100% methyl polysiloxane, 12.5 m × 0.2 mm I.D., 0.33 μm (Supelco equivalent, Equity-1, 28041-U)
oven
60 °C (1 min) to 140 °C (4 min) at 30 °C/min, then to 276 °C at 20 °C/min, 4 min
inj. temp.
splitless (closed 1 min), 270 °C
detector
MS, full scan