- Different co-culture systems have the same impact on bovine embryo transcriptome.
Different co-culture systems have the same impact on bovine embryo transcriptome.
Reproduction (Cambridge, England) (2017-10-07)
A Vitorino Carvalho, E Canon, L Jouneau, C Archilla, L Laffont, M Moroldo, S Ruffini, E Corbin, P Mermillod, V Duranthon
PMID28982934
摘要
During the last few years, several co-culture systems using either BOEC or VERO feeder cells have been developed to improve bovine embryo development and these systems give better results at high oxygen concentration (20%). In parallel, the SOF medium, used at 5% O
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MEM 非必需氨基酸溶液 (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
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庆大霉素 溶液, 10 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture
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牛血清白蛋白 来源于牛血清, lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)
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培养基 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
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BME 氨基酸溶液 50×, Without L-glutamine, sterile-filtered, BioReagent, suitable for cell culture
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肝素 钠盐 来源于猪肠粘膜, Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture
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培养基 199, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture