An HPLC method for the determination of selected amino acids in human embryo culture medium.

An HPLC method for the determination of selected amino acids in human embryo culture medium.

Biomedical chromatography : BMC (2016-07-28)

Petra Drábková, Lenka Andrlová, Roman Kanďár

摘要

A method for the determination of selected amino acids in culture medium using HPLC with fluorescence detection is described. Twenty hours after intra-cytoplasmic sperm injection, one randomly selected zygote was transferred to the culture medium. After incubation (72 h after fertilization), the culture medium in which the embryo was incubated and blank medium was immediately stored at -80°C. Filtered medium samples were derivatized with ortho-phthalaldehyde (naphthalene-2,3-dicarboxaldehyde), forming highly fluorescent amino acids derivatives. Reverse-phase columns (LichroCART, Purospher STAR RP

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