Generation of iPSC line HEL24.3 from human neonatal foreskin fibroblasts.

Generation of iPSC line HEL24.3 from human neonatal foreskin fibroblasts.

Stem cell research (2015-06-22)

Ras Trokovic, Jere Weltner, Timo Otonkoski

摘要

Human iPSC line HEL24.3 was generated from healthy human foreskin fibroblasts using non-integrative reprogramming method. Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using Sendai viruses.

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M3148

Sigma-Aldrich

2-巯基乙醇, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

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2-巯基乙醇, ≥99.0%

D9542

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DAPI, for nucleic acid staining

63689

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2-巯基乙醇, BioUltra, for molecular biology, ≥99.0% (GC)

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431788

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乙二胺四乙酸, 99.995% trace metals basis

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丁酸钠, ≥98.5% (GC)

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