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Merck
  • Human regulatory B cells combine phenotypic and genetic hallmarks with a distinct differentiation fate.

Human regulatory B cells combine phenotypic and genetic hallmarks with a distinct differentiation fate.

Journal of immunology (Baltimore, Md. : 1950) (2014-08-01)
Wenyu Lin, Daniela Cerny, Edmond Chua, Kaibo Duan, June Tai Jing Yi, Nurhidaya Binte Shadan, Josephine Lum, Maud Maho-Vaillant, Francesca Zolezzi, Siew Cheng Wong, Anis Larbi, Katja Fink, Philippe Musette, Michael Poidinger, Sébastien Calbo
摘要

Regulatory B cells (B-reg) produce IL-10 and suppress inflammation in both mice and humans, but limited data on the phenotype and function of these cells have precluded detailed assessment of their contribution to host immunity. In this article, we report that human B-reg cannot be defined based on a phenotype composed of conventional B cell markers, and that IL-10 production can be elicited in both the CD27(+) memory population and naive B cell subset after only a brief stimulation in vitro. We therefore sought to obtain a better definition of IL-10-producing human B-regs using a multiparameter analysis of B cell phenotype, function, and gene expression profile. Exposure to CpG and anti-Ig are the most potent stimuli for IL-10 secretion in human B cells, but microarray analysis revealed that human B cells cotreated with these reagents resulted in only ∼0.7% of genes being differentially expressed between IL-10(+) and IL-10(-) cells. Instead, connectivity map analysis revealed that IL-10-secreting B cells are those undergoing specific differentiation toward a germinal center fate, and we identified a CD11c(+) B cell subset that was not capable of producing IL-10 even under optimal conditions. Our findings will assist in the identification of a broader range of human pro-B-reg populations that may represent novel targets for immunotherapy.

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