跳转至内容
Merck
  • Lipase entrapment in protamine-induced bio-zirconia particles: characterization and application to the resolution of (R,S)-1-phenylethanol.

Lipase entrapment in protamine-induced bio-zirconia particles: characterization and application to the resolution of (R,S)-1-phenylethanol.

Enzyme and microbial technology (2012-05-15)
Jing-Yun Wang, Cui-Li Ma, Yong-Ming Bao, Pin-San Xu
摘要

Lipase from Burkholderia cepacia was encapsulated inside zirconia particles by biomimetic mineralization of K₂ZrF₆ induced with protamine, a natural cationic protein. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR) were employed for the characterization of the novel immobilized lipase. SEM and TEM images showed that both the zirconia particles with and without lipase have good spherical structures with average particle sizes of 150 nm. Fluorescence microscopy demonstrated that the lipase was indeed encapsulated inside the zirconia particles. The maximum immobilization capacity of the zirconia particles was 0.15 units/mg under optimum immobilization conditions. Biochemical characterization showed that the encapsulated lipase could retain most of its initial activity. Compared with free lipase, the encapsulated lipase exhibited improved thermal, pH, and recycling stabilities. After 8 weeks of storage, no substantial loss in catalytic activity was observed for the encapsulated lipase. The conversion of the kinetic resolution of (R,S)-1-phenylethanol with vinyl acetate as acetyl donor catalyzed by zirconia-immobilized lipase reached 49.9% with higher ee(s) of 99.9% under the following optimal conditions: octane as solvent, 0.1M (R,S)-1-phenylethanol, 70 mg immobilized lipase, 180 rpm, 50 °C for 48 h. After 6 cycles (288 h), the conversion and ee(s) were still 43% and 85%, respectively.

材料
货号
品牌
产品描述

Sigma-Aldrich
乙酸乙烯酯, contains 3-20 ppm hydroquinone as inhibitor, ≥99%
登录查看公司和协议定价
货号包装规格是否有货价格
0.2 mL
请联系客服了解存货情况
CA$548.00

如需订购该产品,请通过联系信息 联系默克当地经销商。

Supelco
乙酸乙烯酯, analytical standard
登录查看公司和协议定价
货号包装规格是否有货价格
0.2 mL
请联系客服了解存货情况
CA$548.00

如需订购该产品,请通过联系信息 联系默克当地经销商。

Sigma-Aldrich
1-苯基乙醇, 98%
登录查看公司和协议定价
货号包装规格是否有货价格
0.2 mL
请联系客服了解存货情况
CA$548.00

如需订购该产品,请通过联系信息 联系默克当地经销商。

Sigma-Aldrich
α-甲基苄醇, ≥99%, FCC, FG
登录查看公司和协议定价
货号包装规格是否有货价格
0.2 mL
请联系客服了解存货情况
CA$548.00

如需订购该产品,请通过联系信息 联系默克当地经销商。

Supelco
乙酸乙烯酯 溶液, certified reference material, 5000 μg/mL in acetonitrile
登录查看公司和协议定价
货号包装规格是否有货价格
0.2 mL
请联系客服了解存货情况
CA$548.00

如需订购该产品,请通过联系信息 联系默克当地经销商。

Sigma-Aldrich
乙酸乙烯酯, CP
登录查看公司和协议定价
货号包装规格是否有货价格
0.2 mL
请联系客服了解存货情况
CA$548.00

如需订购该产品,请通过联系信息 联系默克当地经销商。

乙酸乙烯酯, European Pharmacopoeia (EP) Reference Standard
登录查看公司和协议定价
货号包装规格是否有货价格
0.2 mL
请联系客服了解存货情况
CA$548.00

如需订购该产品,请通过联系信息 联系默克当地经销商。