Lung Section Staining and Microscopy.

Bio-protocol (2017-11-25)

Xiaofeng Zhou, Bethany B Moore

摘要

Our protocol describes immunofluorescent staining, hematoxylin and eosin staining and Masson's trichrome staining on lung sections.

材料

货号

品牌

产品描述

Sigma-Aldrich

乙酸, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

盐酸, ACS reagent, 37%

Sigma-Aldrich

氯化铁(III), reagent grade, 97%

Sigma-Aldrich

硫酸铝钾十二水合物, ACS reagent, ≥98%

Sigma-Aldrich

无水柠檬酸粉末, ACS reagent, ≥99.5%

Sigma-Aldrich

苏木精, certified by the BSC

Sigma-Aldrich

伊红 Y, Dye content ~99 %

Sigma-Aldrich

磷酸钠一元, BioXtra, ≥99.0%