Engineering Human Neural Tissue by 3D Bioprinting.

Engineering Human Neural Tissue by 3D Bioprinting.

Methods in molecular biology (Clifton, N.J.) (2018-04-22)

Qi Gu, Eva Tomaskovic-Crook, Gordon G Wallace, Jeremy M Crook

摘要

Bioprinting provides an opportunity to produce three-dimensional (3D) tissues for biomedical research and translational drug discovery, toxicology, and tissue replacement. Here we describe a method for fabricating human neural tissue by 3D printing human neural stem cells with a bioink, and subsequent gelation of the bioink for cell encapsulation, support, and differentiation to functional neurons and supporting neuroglia. The bioink uniquely comprises the polysaccharides alginate, water-soluble carboxymethyl-chitosan, and agarose. Importantly, the method could be adapted to fabricate neural and nonneural tissues from other cell types, with the potential to be applied for both research and clinical product development.

材料

货号

品牌

产品描述

SCC007

Sigma-Aldrich

ReNcell CX人神经祖细胞系, ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.

55467-U

Supelco

Discovery^® Glycan SPE Tube, bed wt. 250 mg, volume 3 mL, pk of 54

55482-U

Supelco

Discovery^® Glycan SPE Tube, bed wt. 500 mg, volume 6 mL, pk of 30

55508-U

Supelco

Discovery^® Glycan SPE Bulk Packing, pkg of 50 g

55487-U

Supelco

Discovery^® Glycan SPE Tube, bed wt. 1 g, volume 12 mL, pk of 20

55505-U

Supelco

Discovery^® Glycan SPE Tube, 5 g, volume 60 mL, pk of 16