技術
HPLC: suitable
測試參數
column: SeQuant ZIC-HILIC, PEEK, 150 x 0.3 mm, 5 μm, 200 Å (1.50481.0001)
mobile phase: [A] 0.25% (v/v) formic acid with Milli-q-water; [B] 0.25% (v/v) formic acid in acetonitrile; (10:90, A:B)
gradient: Initial composition: 90% B and 10% A. Linear gradient from 0-40 min
flow rate: 5 μL/min
detector: MicroMass Ultima-QTOF, Needle voltage: 3.1 kV, Cone voltage: 45V (collision energy: 10V; Scan range: m/z 400-1800; 1.5 s cycle time)
injection: 1 μL of 10 pmole digested sample injected in mobile phase
適合性
application for HPLC
應用
life science and biopharma
法律資訊
SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
T34 (R)ZVSP(-)
T11 (R)YPILPEYLQZVK€
T26 (K)VYLPR(M)
T16 (K)GLWEK(A)
T12 (K)ELYR(G)
T10 (R)LYAEER(Y)
T20 (R)VASMASEK(M)
T32 (R)ADHPFLFZ1K(H)
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