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Merck

G005908

HPLC Analysis of Aflatoxins on Ascentis® Express C18

application for HPLC

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About This Item

技術

HPLC: suitable

測試參數

column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] water; [B] methanol; [C] acetonitrile; (720:120:120, A:B:C) containing 0.780 g/L potassium bromide, 230 μL/L nitric acid
flow rate: 0.400 mL/min
pressure: 4568 psi (315 bar)
column temp.: 35 °C
detector: FLD excitation = 360 nm, emission = 440 nm
injection: 20 μL
sample: 200 μL spiked 84:16 acetonitrile:di water in 880 μL di water, Spike concentrations: B1 = 16 ppb; B2 = 4 ppb; G1 = 16 ppb; B2 = 4 ppb

適合性

application for HPLC

應用

food and beverages

分析報告

Aflatoxins are a complex of closely related toxins produced as secondary metablolites of fungi. They are found in grains and nuts such as corn, wheat, or peanuts. They are produced by aspergillus flavus and a. Parasiticus. Members of the group include aflatoxin b1, aflatoxin b2, aflatoxin g1, aflatoxin g2, aflatoxin m1, and aflatoxin m2.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

说明

    Aflatoxin G2
    Aflatoxin G1
    Aflatoxin B2
    Aflatoxin B1

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