G005683

HPLC Analysis of Caffeine and Taurine on Ascentis^® Express HILIC Column using Evaporative Light Scattering Detection (ELSD, Fast Analysis)

application for HPLC

属性

• 技术: HPLC: suitable
• 试验参数: column: Ascentis Express HILIC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U); mobile phase: [A] 100 mM ammoniumformate, pH 3.0 with formic acid; [B] acetonitrile; (15:85, A:B); flow rate: 0.6 mL/min; column temp.: 25 °C; detector: ELSD, 55 °C, 3.5 bar nitrogen; injection: 2 μL; sample: 1000 μg/mL in 10:90, water: acetonitrile
• 适用性: application for HPLC
• 应用: vitamins, nutraceuticals, and natural products

说明

分析说明

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

Caffeine
Taurine