技术
HPLC: suitable
试验参数
column: Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)
mobile phase: [A] water, acetonitrile, 90:10; [B] acetonitrile
gradient: 0% B held for 0.5 min; to 15% B in 1 min; held at 15% B for 6 min
flow rate: 0.3 mL/min
pressure: 192 bar (2780 psi)
column temp.: 30 °C
detector: UV, 218 nm
injection: 10 μL
sample: 5 mg/L ea. in 95:5, water:acetonitrile
适用性
application for HPLC
应用
agriculture
food and beverages
分析说明
The trichothecene vomitoxins of Fusarium are significant plant pathogens of grain and forage crops that can lead to anorexic effects with livestock. This application demonstrates resolution of deoxynivalenol and three of its analogs.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Nivalenol hydrate
15-Acetyldeoxynivalenol solution
3-Acetyldeoxynivalenol
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