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描述
AF-Chelate-650M
產品線
TOYOPEARL®
形狀
liquid
包裝
bottle of 25 mL
製造商／商標名
Tosoh TOYOPEARL® AF, 14475
標籤範圍
25-45 μeq/mL ligand density
參數
3 bar max. pressure
技術
HPLC: suitable
基質
hydroxylated methacrylic polymer
基質活性組
IDA phase, iminodiacetic acid
粒徑
65 μm
工作pH值
2-12
分離技術
affinity
儲存溫度
2-8°C
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一般說明
Toyopearl AF-Chelate-650 resin is derivatized with iminodiacetic acid for immobilized metal affinity chromatography or IMAC.IMAC works primarily by binding to histidine and free cysteine containing sequences of proteins or peptides.
應用
TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.
規格
Clean in place with 0.5 M NaOH or 0.1 M HCl.
外觀
Shipped in 20% (v/v) ethanol.
法律資訊
Toyopearl is a registered trademark of Tosoh Corporation
訊號詞
Warning
危險聲明
危險分類
Flam. Liq. 3
水污染物質分類（WGK）
WGK 1
閃點（°F）
103.1 °F
閃點（°C）
39.5 °C
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分析证书(COA)
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S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;
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