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Merck
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567523-U

Supelco

Discovery® HS F5 (5 µm) HPLC Columns

L × I.D. 25 cm × 21.2 mm, HPLC Column

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery ® HS F5 HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 21.2 mm

材料

stainless steel column

agency

suitable for USP L43

產品線

Discovery®

特點

endcapped

製造商／商標名

Discovery®

包裝

1 ea of

標籤範圍

12% Carbon loading

參數

≤70 °C temp. range
400 bar pressure (5801 psi)

技術

HPLC: suitable

長度 × 內徑

25 cm × 21.2 mm

表面積

300 m2/g

表面覆盖率

4 μmol/m2

雜質

<10 ppm metals

基質

silica gel, high purity, spherical particle platform
fully porous particle

基質活性組

PFP (pentafluorophenyl) phase

粒徑

5 μm

孔徑

120 Å

工作pH值範圍

2-8

應用

food and beverages

分離技術

reversed phase

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相关类别

一般說明

将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则：
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来，使得大部分的 C18 方法可轻松转换到 F5 上。

特點和優勢

  • 独特的选择性
  • 保留能力与 C18 柱类似（有时需要洗脱能力更强的流动相）
  • 峰形极佳
  • 稳定性好、低流失，适用于 LC-MS 分析
  • 粒径范围从 3μm 到 10μm 可供选择

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法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Choose from one of the most recent versions:

分析证书(COA)

Lot/Batch Number
290839-01
281966-01
267860-01
259541-01
259512-01

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Koji Baba et al.
Journal of chromatography. A, 1354, 109-116 (2014-06-21)
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Umberto M Battisti et al.
Journal of chromatography. A, 1443, 152-161 (2016-03-30)
A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.
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