ZRB2789

Anti-CD11a Antibody, clone 2J15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: Anti-FLT4 Antibody, clone 2F4 ZooMAb^® Rabbit Monoclonal, Integrin alpha-L (UniProt: P20701, also known as CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain
• UNSPSC代码: 12352203
• NACRES: NA.43

属性

• 生物来源: rabbit
• 质量水平: 200
• 重组: expressed in HEK 293 cells
• 偶联物: unconjugated
• 抗体形式: purified antibody
• 抗体产品类型: primary antibodies
• 克隆: 2J15, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 产品线: ZooMAb^® learn more
• 表单: lyophilized
• 分子量: calculated mol wt 128.77 kDa; observed mol wt ~180 kDa
• 纯化方式: using Protein A
• 种属反应性: human
• 包装: antibody small pack of 25
• 环保替代产品特性: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 增强验证: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技术: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同位素/亚型: IgG
• 表位序列: C-terminal half
• Protein ID登记号: NP_002200
• UniProt登记号: P20701
• 环保替代产品分类: Aligned
• 运输: ambient
• 储存温度: 2-8°C
• 基因信息: human ... ITGAL(3683)

说明

一般描述

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特异性

Clone 2J15 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects CD11a. It targets an epitope within 23 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal half of human CD11a.

应用

Quality Control Testing

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD11a in THP-1 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CD11a in lysates from Jurkat cells and Human spleen tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD11a in Human spleen tissue sections.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected CD11a in one million Human peripheral blood mononuclear cells (PBMC)..

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD11a in MV4-11 cells.

Affinity Binding Assay: A representative lot of this antibody bound in CD11a peptide with a KD of 2.9 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

目标描述

Integrin alpha-L (UniProt: P20701; also known as CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, LFA-1A, Leukocyte function-associated molecule 1 alpha chain, CD11a) is encoded by the ITGAL (also known as CD11A) gene (Gene ID: 3683) in human. Integrins are heterodimeric, integral membrane proteins composed of a variable -subunit and a conserved β-subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various and β-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the -subunit. CD11a is a single-pass type I membrane glycoprotein that noncovalently associates with β2 integrin to forms a heterodimer and serve as a receptor for IC

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

储存及稳定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable