生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1G5, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 22.18 kDa
observed mol wt ~18 kDa
純化經由
using Protein A
物種活性
human, mouse, rat
物種活性（以同源性預測）
monkey, chimpanzee
包裝
antibody small pack of 25
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
技術
affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
儲存溫度
2-8°C
一般說明
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1G5 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects GPx4. It targets an epitope within 22 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human GPX4.
應用
Quality Control Testing
Evaluated by Western Blotting in U-937 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected GPx4 in U-937 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000-10,000 dilution from a representative lot detected GPx4 in lysate from mouse liver and rat testis tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GPx4 in human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound GPx4 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in U-937 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected GPx4 in U-937 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000-10,000 dilution from a representative lot detected GPx4 in lysate from mouse liver and rat testis tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GPx4 in human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound GPx4 peptide with at least ten thousand-fold (10,000X) higher than with non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Phospholipid hydroperoxide glutathione peroxidase, cytosolic (UniProt: P36969; also known as PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4) is encoded by the GPX4 gene (Gene ID: 2879) in human. The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. This selenocysteine is crucial for the enzyme′s catalytic activity. Sec is encoded by the UGA codon, which normally signals translation termination. Three functionally distinct isozymes of GPx4 have been described and are known as mGPx4 (mitochondrial), cGPx4 (cytosolic), and nGPx4 (nuclear). They originate from a single GPX4 gene. The cytosolic isoform is ubiquitously expressed at moderate levels in most mammalian cells. On the other hand, mitochondrial and nuclear isoforms are overexpressed in spermatoid cells. GPx4 is a considered to be a ′moonlighting′ protein because of its ability to serve dual functions as a peroxidase, that has been implicated in anti-oxidative defense, gene expression regulation, and programmed cell death, and as a structural protein in spermatogenesis. Mutations or deficiencies in GPX4 are associated with several diseases. One of the most severe conditions linked to GPX4 deficiency is Sedaghatian-type spondylometaphyseal dysplasia (SMDS), a rare genetic disorder characterized by skeletal abnormalities, neurological impairment, and early infant death. Homozygous GPx4-deficient mice are reported to die utero before E7.5. Similarly, homozygous mice expressing catalytically inactive GPx4 variants (Sec46Ala/Ser mutant) do not survive beyond the 7th day of embryogenesis. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Borchert, A., et al (2010). Immunol. Lett. 133 (2); 85-93).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
重構
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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