推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
2B23, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
純化經由
using Protein A
物種活性
human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
表位序列
N-terminal
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
儲存溫度
2-8°C
基因資訊
human ... ADRB2(154)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 2B23 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects β2-Adrenergic Receptor. It targets an epitope within 19 amino acids in the first extracellular domain from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 19 amino acids from the first extracellular domain of human β2-Adrenergic Receptor.
應用
Quality Control Testing
Evaluated by Western Blotting in ACHN cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected β2-Adrenergic Receptor in ACHN cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected β2-Adrenergic Receptor in HepG2 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound β2-Adrenergic Receptor peptide with a KD of 1.2 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected β2-Adrenergic Receptor in human heart tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected β2-Adrenergic Receptor in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in ACHN cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected β2-Adrenergic Receptor in ACHN cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected β2-Adrenergic Receptor in HepG2 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound β2-Adrenergic Receptor peptide with a KD of 1.2 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected β2-Adrenergic Receptor in human heart tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected β2-Adrenergic Receptor in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Beta-2 adrenergic receptor (UniProt: P07550; also known as Beta-2 adrenoreceptor, Beta-2 adrenoceptor) is encoded by the ADRB2 (also known as ADRB2R, B2AR) gene (Gene ID: 154) in human. β-adrenergic receptors are activated by the catecholamines (norepinephrine and epinephrine) and are family of the 7-transmembrane superfamily of receptors. Three distinct β adrenergic receptors have been described: β1, β2, and β3. They are identified in cardiac, airway smooth muscle, and adipose tissue, respectively and display about 65% to 70% homology. β2-adrenergic receptor is a multi-pass membrane glycoprotein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. It mediates the catecholamine-induced activation of adenylate cyclase that results in the production of cyclic AMP and subsequent activation of protein kinase A (PKA). Most of the downstream effects of β-adrenergic receptor activation are due to the action of PKA. Activation of β2 receptor causes smooth muscle relaxation, peripheral vasodilation, and subsequent hypotension. It is palmitoylated at cysteine 341, which reduces accessibility of phosphorylation sites by anchoring the receptor to the plasma membrane. Agonist stimulation of β-adrenergic receptors promote de-palmitoylation and allows phosphorylation of serine 345 and 346. Phosphorylation of β2 receptor by PKA and β-adrenergic receptor kinase (BARK) mediates homologous desensitization of the receptor. PKA-mediated phosphorylation appears to facilitate phosphorylation by BARK. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity™, reproducibility, and stability over conventional monoclonals. (Ref.: Johnson, M. (2006). J. Allergy Clon. Immunol. 117(1); 18-24; Moffett, S., et al. (2001). J. Neurochem. 76(1); 269-279; O′Dowd, BF., et al. (1989). J. Biol. Chem. 264(13); 7564-7569).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
重構
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
Affinity is a trademark of Mine Safety Appliances Co.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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