ZRB2158

Anti-IRF-5 Antibody, clone 1L11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: Interferon regulatory factor 5
• 分類程式碼代碼: 12352203
• NACRES: NA.43

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1L11, recombinant monoclonal
• 描述: 1L11 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 56.04 kDa; observed mol wt ~56 kDa
• 純化經由: using Protein A
• 物種活性: human, mouse
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal half
• Protein ID登錄號: NP_001092097
• UniProt登錄號: Q13568
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... IRF5(3663)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone IL11 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Interferon regulatory factor 5 (IRF-5). It targets an epitope within 19 amino acids from the N-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Interferon regulatory factor 5 (IRF-5).

應用

Quality Control Testing

Evaluated by Western Blotting in LNCap cell nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IRF-5 in LNCap cell nuclear extract.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected IRF-5 in THP-1 and NIH3T3 cell nuclear extracts.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected IRF-5 in one million THP-1 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IRF-5 in THP-1 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected IRF-5 in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound IRF-5 peptide with a KD of 4.9 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in LNCap cell nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IRF-5 in LNCap cell nuclear extract.

標靶描述

Interferon regulatory factor 5 (UniProt: Q13568; also known as IRF-5) is encoded by the IRF5 gene (Gene ID: 3663) in human. The interferon regulatory factors (IRFs) are a family of nine transcription factors that regulate pathogen-induced innate and acquired immune responses. In unstimulated cells they reside in the cytoplasm. IRF-5 plays a critical role in innate immunity by activating expression of type I interferons (IFN- and β) and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8, and TLR9. It regulates the transcription of IFN genes (IFNA and IFNB) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It is shown to be a key transcription factor regulating the interferon response during SARS-CoV-2 infection. It is maintained as a monomer in an autoinhibited state and undergoes homodimerization and activation following its phosphorylation on serine 451 and 462 in the C-terminal autoinhibitory region. It has been reported that phosphorylation of serine 451 relieves the autoinhibitory conformation, and phosphorylation of serine 462 stabilizes the IRF-5 homodimer. In its phosphorylation and activation scheme, the innate adapter protein TASL (TLR adaptor interacting with SLC15A4 on the lysosome) recruits IRF-5, which is then phosphorylated by IKK-β at serine 462 that allows IRF-5 dissociation from the adapter protein followed by its dimerization and nuclear translocation. Mutations in IRF5 gene have been linked to systemic lupus erythematosus 10 (SLEB10), rheumatoid arthritis, and irritable bowel syndrome. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Thompson, CD., et al. (2018). Front. Immunol. 9; 2622; Lopez-Pelaez, M., et al. (2014). Proc. Natl Acad. Sci. USA. 111(49); 17432-17437; Foreman, HCC., et al. (2012). PLoS One. 7(3); e33098).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable