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生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1E18, recombinant monoclonal
描述
1E18 Clone
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 38.46 kDa
observed mol wt ~Target molecular weight 28 and 34 kDa
純化經由
using Protein A
物種活性
human, mouse
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
表位序列
N-terminal half
Protein ID登錄號
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... MFF(56947)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1E18 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Mitochondrial fission factor (MFF). It targets an epitope within 25 amino acids from the N-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal half of human Mitochondrial fission factor (MFF).
應用
Quality Control Testing
Evaluated by Western Blotting in T47D cell lysates.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MFF in T47D cell lysates.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MFF in lysates from Human Brain and MOLT4 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MFF in human cerebral cortex tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound MFF peptide with a KD of 8.8 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MFF in C2C12 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in T47D cell lysates.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MFF in T47D cell lysates.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MFF in lysates from Human Brain and MOLT4 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MFF in human cerebral cortex tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound MFF peptide with a KD of 8.8 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MFF in C2C12 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Mitochondrial fission factor (UniProt: Q9GZY8; also known as MFF) is encoded by the MFF (also known as C2orf33, AD030, AD033, GL004) gene (Gene ID: 56947) in human. MFF is a single-pass type IV homodimeric membrane protein that has a cytoplasmic domain (aa 1-322), a helical domain (aa 323-340), and a mitochondrial intermembrane domain (aa 341-342). It is highly expressed in heart, kidney, liver, brain, muscle, and stomach. It is a homodimeric protein but may also interact with other proteins. It plays a role in mitochondrial and peroxisomal fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. It is also suggested to be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles. MFF is shown to be an essential factor for mitochondrial recruitment of Drp1 during mitochondrial fission in mammalian cells and its down-regulation inhibits mitochondrial recruitment of Drp1 and induces mitochondria elongation. Exogenous expression of MFF shown to induce extensive mitochondrial fission. Abnormal activation of mitochondrial fission may drive the impairment of mitochondrial metabolic function that leads to inhibition of cancer stem cell propagation, and the activation of quiescence programs. Mutations in MFF gene can cause a form of encephalopathy that is characterized by delayed psychomotor development, severe hypotonia with inability to walk, and microcephaly. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sanchez-Alvarez, R., et al. (2020). Front. Oncol. 10: 1776; Otera, H., et al. (2010). J. Cell Biol. 191 (6); 1141 1158; Gandre-Babbe, S., and van der Bliek, AM. (2008). Mol. Biol. Cell. 19(6): 2402-2412).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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