推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1F17, recombinant monoclonal
描述
1F17 Clone
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 13.95 kDa
observed mol wt ~24 kDa
純化經由
using Protein A
物種活性
rat, human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
ubiquitination (Lys120)
基因資訊
human ... H2BC3(3018)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1F17 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Histone H2B ubiquitylated on lysine 120.
免疫原
KLH-conjugated linear peptide corresponding to 13 amino acids surrounding ubiquitylated lysine 120 of human Histone 2B.
應用
Quality Control Testing
Evaluated by Western Blotting in HeLa cell acid extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ubiquityl Histone H2B-Lys120 in HeLa cell acid extract.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ubiquityl Histone H2B-Lys120 in HL-60 cell and Rat2 cell acid extracts .
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Ubiquityl Histone H2B-Lys120 in human liver tissue sections.
Peptide Inhibition Assay: Target band detection in a lysate from HeLa cell acid extract was prevented by pre-blocking of a representative lot with Ubiquityl Histone H2B-Lys120 peptide, but not the corresponding non- Ubiquityl H2B peptide.
Affinity Binding Assay: A representative lot of this antibody bound Ubiquityl Histone H2B-Lys120 peptide with a KD of 3.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa cell acid extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ubiquityl Histone H2B-Lys120 in HeLa cell acid extract.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ubiquityl Histone H2B-Lys120 in HL-60 cell and Rat2 cell acid extracts .
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Ubiquityl Histone H2B-Lys120 in human liver tissue sections.
Peptide Inhibition Assay: Target band detection in a lysate from HeLa cell acid extract was prevented by pre-blocking of a representative lot with Ubiquityl Histone H2B-Lys120 peptide, but not the corresponding non- Ubiquityl H2B peptide.
Affinity Binding Assay: A representative lot of this antibody bound Ubiquityl Histone H2B-Lys120 peptide with a KD of 3.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Histone H2B type 1-B (UniProt: P33778; also known as H2B-clustered histone 3, Histone H2B.1, Histone H2B.f (H2B/f) is encoded by the H2BC3 (also known as H2BFF, HIST1H2BB) gene (Gene ID: 3018) in human. Histone H2B is a core component of nucleosome that wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B features a main globular domain and a long N terminal tail. Histone H2B mono-ubiquitinated on lysine120 during transcriptional activation by RAD6E2 protein and is associated with the transcribed regions of highly expressed genes. It is a key histone modification that plays critical roles in gene transcriptional regulation and higher order chromatin organization. Mono-ubiquitination of histone H2B stimulates transcriptional elongation by facilitating FACT-dependent chromatin remodeling. De-ubiquitination of histone H2B lysine120 by USP22 is a required step in transcriptional activation. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, S., et al. (2012). Cell Research. 22(9); 1402-1405; Zhu, B., et al. (2006). Mol. Cell. 20(4); 601-611).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 产品选型工具.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门