生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1K9, recombinant monoclonal
描述
1K9 Clone
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 192.22 kDa
observed mol wt ~230 kDa
純化經由
using Protein A
物種活性
human
物種活性（以同源性預測）
porcine, monkey
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... RICTOR(253260)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1K9 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Rictor. It targets an epitope within 22 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal region of human Rictor.
應用
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Rictor in HEK293 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rictor in MDA-MB-231 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound Rictor with a KD of 1.1 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Rictor in human colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Rictor in HEK293 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rictor in MDA-MB-231 cell lysate.
Affinity Binding Assay: A representative lot of this antibody bound Rictor with a KD of 1.1 x 10-6 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Rictor in human colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Rapamycin-insensitive companion of mTOR (UniProt: Q6R327; also known as AVO3 homolog, hAVO3, Rictor) is encoded by the RICTOR (also known as KIAA1999) gene (Gene ID: 253260) in human. The mammalian target of rapamycin (mTOR) is an evolutionarily conserved serine/threonine kinase that acts as a nutrient sensor and regulates growth factor signal transduction and cellular metabolism. It is an important regulator of protein synthesis, translation initiation, transcription of ribosomal proteins, and the synthesis of rRNA and tRNA. Cellular mTOR is composed of two large protein complexes, known as mTORC1 and mTORC2. The mTORC1 complex is sensitive to rapamycin and consists of mTOR, Raptor, and mLST8 and two negative regulators, PRAS40 and DEPTOR. The Raptor unit serves as a scaffolding protein to bridge the interaction between mTOR and its substrates. The mTORC2 complex that is insensitive to rapamycin, contains mTOR, Rictor, Sin1, and mLST8, Protor, Hsp70, and DEPTOR. This complex plays a role in actin cytoskeleton organization and phosphorylates Akt on Ser473 to fully activate it. Rictor is shown to be essential for Akt phosphorylation at Ser 473. Insensitivity of mTORC2 to rapamycin is reported to be due to the presence of Rictor. Rictor can be phosphorylated at Thr 1135 by ribosomal protein S6 kinase B1 (RPS6KB1), which leads to inhibition of mTORC2 and Akt1 signal transduction. Alterations in RICTOR gene have been identified in breast, pancreatic, colon, and lung cancers. Rictor is also involved in tumor progression by regulating the tumor microenvironment through angiogenesis and remodeling of the stroma. In pancreatic tumors, blockage of Rictor is shown to inhibit HIF-1 expression and reduce the level of VEGF-A. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jebali, A., and Dumaz, N. (2018). Mol. Cancer. 17; Article 39; Schmidt, KM., et al. (2017). Oncotarget. 8(15); 24491-24505).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
商品
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
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