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生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
2C13, monoclonal
recombinant monoclonal
描述
2C13 Clone
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt ~11.32 kDa
observed mol wt ~8 kDa
物種活性
rat, mouse, human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
特異性
Clone 2C13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects G0/G1 switch protein 2 (G0S2).
免疫原
GST/His-tagged full-length recombinant human G0/G1 switch protein 2 (G0S2).
應用
Quality Control Testing
Evaluated by Western Blotting in Rat Heart tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected G0S2 in Rat Heart tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected G0S2 in lysates from human heart, mouse heart, and A7R5 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected G0S2 in rat heart tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound G0S2 protein with a KD of 2.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Rat Heart tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected G0S2 in Rat Heart tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected G0S2 in lysates from human heart, mouse heart, and A7R5 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected G0S2 in rat heart tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound G0S2 protein with a KD of 2.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-G0S2, clone 2C13 ZooMAb®, Cat. No. ZRB1813, is a recombinant Rabbit monoclonal antibody that specifically targets G0S2 and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
標靶描述
G0/G1 switch protein 2 (UniProt: P27469; also known as G0/G1 switch regulatory protein 2, Putative lymphocyte G0/G1 switch gene, G0S2) is encoded by the G0S2 gene (Gene ID: 50486) in human. G0S2 is a highly conserved, mitochondrial protein that is widely expressed in vertebrate species. High expression of G0S2 is reported in peripheral blood cells, skeletal muscle, heart, liver and kidney. It is multifaceted protein that is involved in cell proliferation, apoptosis, inflammation, metabolism, and carcinogenesis. The G0S2 gene is reported to be hypermethylated in several human cancer cell lines. Its promoter region contains potential binding sites for AP1, AP2, AP3, and Nuclear factor of activated T cells (NFAT). The G0S2 protein is shown to contain two -helices that are separated by a hydrophobic sequence with the potential to generate turns and assume a β-sheet conformation. It also has several putative phosphorylation sites for protein kinase C (PKC) and casein kinase II. G0S2 can interact with Bcl2 and block the formation of Bcl2-Bax heterodimer to block apoptosis. In fat tissue it acts as a molecular brake on triglyceride catabolism. G0S2 is reported to be a major selective inhibitor of adipose triglyceride lipase (AGTL) and its hydrolase activity. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Heckmann, BL., et al. (2013). Biochim. Biophys. Acta. 1831(2); 276-281; Heckmann, BL., et al. (2016). PLoS ONE 11(6); e0156742).
外觀
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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